Hu antibodies

Indication

Suspicion of paraneoplastic syndrome.

Method

Immunoblot and indirect immunofluorescence (IIF). For IIF test it is applied a combination of fixed tissues from the nerve, cerebellar, intestinal tissue and pancreas originating from monkeys.

According to guidelines, anti-neuronal antibodies should be detected by at least two independent methods. At Wieslab IIF and immunoblot are used. According to European guidelines, a positive result should be obtained with both methods to be reliable. According to the supplier the assay sensitivity is 100% and specificity is 100%.
Immunoblot and IIF are accredited analysis for serum samples – read more at the Quality page.

Result

Serum: The result is reported as negative, borderline or positive with a titer and blot intensity.

CSF: The result is reported as negative, borderline or positive

Interpretation

Antibodies against Hu are also called ANNA-1 (anti-neuron-specific cell nuclear antibodies) and occur in paraneoplastic subacute sensory neuronopathy and/or paraneoplastic encephalomyelitis. The antibody prevalence is strongly associated to small cell lung cancer but can also be seen in other tumor types (e.g. breast cancer, Hodgkin's lymphoma, melanoma, ovarian cancer, testicular cancer, thymoma). Neurological symptoms can precede cancer diagnosis by up to 5 year.
 
References: PMID: 15342008: 15258215: 21747075: 22448032

Accredited analysis – read more at the Quality page.

Read more about sampling

Packages and other tests

View other packages Lambert-Eatons myastena syndrom (LEMS) (563), Paraneoplastic Syndromes and Limbic Encephalitis (560, 561 and 562). View other tests AMPA receptor 1 and 2 (GluR1 and GluR2) antibodies, Amphiphysin antibodies, CASPR2 antibodies, CV2/CRMP5 antibodies, DPPX (dipeptidyl aminopeptidase-like protein 6) antibodies, GABA B receptor antibodies, LGI-1 antibodies, Ma antibodies, NMDA receptor antibodies, Purkinje cell antibodies (Tr, Trotter antigen), Recoverin antibodies, Ri (Nova 1/ ANNA-2) antibodies, SOX1 antibodies, Titin antibodies, VGKC (voltage gated potassium channel) antibodies, Yo (PCA-1, Purkinje cells antigen 1) antibodies, Zic4 antibodies

Clinical studies