Amphiphysin antibodies

Indication

Suspicion of paraneoplastic syndrome (especially Stiff person syndrome) and autoimmune encephalitis.

Method

Immunoblot and indirect immunofluorescence (IIF). For IIF test it is applied a combination of fixed tissues from the nerve, cerebellar, intestinal tissue and pancreas originating from monkeys.

According to guidelines, anti-neuronal antibodies should be detected by at least two independent methods. At Wieslab, IIF and immunoblot are used. According to European guidelines, a positive result should be obtained with both methods to be reliable. According to the supplier the assay sensitivity is 100% and specificity is 100%.

Immunoblot and IIF are accredited analysis for serum samples – read more at the Quality page.

Result

Serum: The result is reported as negative, borderline or positive with a titer and blot intensity.

CSF: The result is reported as negative, borderline or positive.

Interpretation

Antibodies against Amphiphysin occur in patients with paraneoplastic variant Stiff person syndrome but can also be seen in autoimmune encephalitis. Antibodies against Amphiphysin are associated with breast cancer and small cell lung cancer. Neurological symptoms may precede the diagnosis of cancer with up to five years.

Referenser 

PMID: 15342008; 15258215; 21747075; 22448032.

Read more about sampling

Packages and other tests

View other packages Lambert-Eatons myastena syndrom (LEMS) (563), Paraneoplastic Syndromes and Limbic Encephalitis (560, 561 and 562), Stiff person syndrome/PERM (568). View other tests AMPA receptor 1 and 2 (GluR1 and GluR2) antibodies, ANNA-3 antibodies, CV2/CRMP5 antibodies, GABA B receptor antibodies, GAD antibodies, Glycine receptor (GlyR) antibodies, Hu antibodies, LGI-1 antibodies, Ma antibodies, NMDA receptor antibodies, Purkinje cell antibodies (PCA 2), Purkinje cell antibodies (Tr, Trotter antigen), Ri (Nova 1/ ANNA-2) antibodies, Ryanodine receptor antibodies, SOX1 antibodies, Titin antibodies, VGCC (voltage-gated calcium channel) antibodies, VGKC (voltage gated potassium channel) antibodies, Yo (PCA-1, Purkinje cells antigen 1) antibodies, Zic4 antibodies

Clinical studies